THE FIRST PORTABLE LABEL-FREE MICROPLATE READER
The Labelfree 96 is the first portable label-free reader. Based on the Corning® Epic® System it is designed to read out 96-well microplates and to perform a wide range of cellular assays. In combination with a wireless connection and an integrated battery, the compact construction of the Labelfree 96 makes it entirely mobile and easy to integrate in liquid-handling-systems.
The Labelfree 96 is designed to read out the Corning® Epic® 96-well cellular assay microplates. These microplates are ANSI /SBS standard 96-well microplates with patented optical biosensors integrated into each well.
The standard uncoated cellular micro-plate is tissue-culture-compatible and enables the attachment and normal growth of adherent cells, including native cells, recombinant/engineered cell lines, and primary cells. The surface of each Epic® fibronectin-coated cellular assay microplate enables attachment and growth of weakly adherent cells.
Label-based techniques have been used for revealing biological interactions for a long time. But there are issues that impact the bioactivity of the labeled biological system. Furthermore, the potential incompatibility with living cells is a clear disadvantage of label-based techniques. In comparison, label-free detection is the more native, simpler and more physiological alternative. It delivers real-time data on biological process, which is especially important for living cell assays.
Main features of label-free
The technology of the Labelfree 96 reader is based on a patented optical reflection measurement. Each well contains its own individual detection unit, consisting of light emitting diodes (LEDs), where Photodiodes and a sophisticated optic is implemented. These detection units use the intensity of the reflection to measure even smallest mass changes on the surface of the Epic® biosensor. The detectable area is defined by the penetration depth of the light out of the sensor, which is about 200 nm.
With the Labelfree 96 reader complex cellular mechanisms both of endogen and of recombinant target molecules can be characterized and analyzed. The origin of the signal can be either a cellular mass change or a dynamic mass redistribution (DMR). Experiments can be observed from the very beginning of the experiment up to several days.
In a proliferation assay the number of cells is proportional to the mass on the sensor surface and is monitored in real time with the Labelfree 96. The microplate and the Labelfree96 can be placed in the incubator with a wireless connation to the computer via Bluetooth. In the exemplary proliferation assay A431 cells were added to the wells and cell growth is recorded at 37˚C.
Like in many other signaling assays, during a GPCR assay dynamic mass redistribution (DMR) is detected by the Labelfree 96. In this example a bradykinin assay with A431 cells is performed at room temperature. The obtained EC50 was 0.45 nM, which is similar to results from the literature.